2013-05-08

2011-04-01

Trofile The TrofileTM HIV co-receptor tropism assay (Monogram Biosciences) (Figure 1) determines whether a patient's viral population is CCR5 (R5), CXCR4 (X4) or Flipr Potassium assay kit measures functional activity of ligand- and voltage- gated potassium channels. this assay kit exploits the permeability of thallium ions Listen to Dr. Bharathi Vennapusa discuss various topics related to NTRK gene fusion testing methodologies and recommendations for identifying patients with IHC Feb 9, 2021 The Seahorse XFe96 extracellular flux analyzer is used to detect, in real time, effects of compounds on oxygen consumption rate (OCR) and The software supports direct binding and inhibition assays, which enables measurement of active concentration. Calibration-free concentration analysis ( CFCA) Jan 9, 2012 solutions (with the amine coupling chips), the ProteOn chips provide high- quality, reproducible SPR assays for a broad range of applications. Cryo electron microscope imaging, automated with the Krios G4 Cryo-TEM, a highly stable 300 kV TEM platform suited for automated single particle analysis, Elabscience specializes in the self-developed Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method), strict quality control, fast cell-based phenotypic assay [14, 15].

Coreceptor tropism assay

Strategies to assess viral tropism may be divided into phenotypic and genotypic assays; registrative trials of CCR5 inhibitors used phenotypic assay, but recently genotypic ones 2013-05-08 CORECEPTOR TROPISM ASSAYS HIV-1 can attach to human cells either by using the CCR5 coreceptor, the CXCR4 coreceptor, or both (dual/mixed). Tropism testing determines how the virus can attach to the cells in a given patient. Possible tropism results are R5, DM, X4, and X4 near the limit of detection (NLOD). Why does my patient’s tropism matter? A co-receptor tropism assay should be performed whenever the use of a CCR5 co-receptor antagonist is being considered (AI).

Coreceptor tropism is defined as the ability of a particular HIV-1 virus to infect a target cell using a specific coreceptor. HIV requires two binding events to enter into a cell. It must first bind CD4 and then, secondly, a chemokine receptor. Tropism is a label given to the virus that describes which chemokine receptor the virus is using.

J Infect Dis 2009; 200:1724. Monogram Biosciences Introduces New Co-Receptor Tropism Assay, Trofile, Availability August 20, 2007 Dear Friends, On August 6, 2007, the FDA approved Selzentry (maraviroc), the first in a new oral class of HIV medicines in more than ten years.

The original tropism assay was used to determine viral coreceptor use for ACTG A5211 participants at study screening; entry; day 14; weeks 8, 24, and 48; and, if applicable, at the time of confirmation of virologic failure, which was defined as a confirmed plasma HIV-1 RNA level of <1 log 10 copies/mL (Ultrasensitive Roche Amplicor HIV-1 Monitor assay) below the baseline level noted at or

Genotypic and phenotypic tropism assays of plasma HIV-1 are available as clinical tests and are recommended in US and European guidelines [7, 8, 9]. However, there is now increased emphasis on using HIV-1 proviral DNA (pvDNA) to ascertain tropism. Phenotypic testing, such as the Trofile assay, is primarily based on ex vivo determination of tropism in established coreceptor expressing cell lines, and has been the gold standard for tropism Test ID BLOD1301 Trofile Co-Receptor Tropism Assay Useful For To determine the co-receptor tropism (CCR5, CXCR4, or dual/mixed) of a patient's HIV-1 strain for selection of CCR5 co-receptor antagonist therapy, when a patient's HIV-1 viral load is ≥ 1,000 copies/mL. The original tropism assay was used to determine viral coreceptor use for ACTG A5211 participants at study screening; entry; day 14; weeks 8, 24, and 48; and, if applicable, at the time of confirmation of virologic failure, which was defined as a confirmed plasma HIV-1 RNA level of <1 log 10 copies/mL (Ultrasensitive Roche Amplicor HIV-1 Monitor assay) below the baseline level noted at or “Coreceptor tropism testing is recommended in patients who exhibit virologic failure on a CCR5 inhibitor.” [Evidence level BIII] “A phenotypic tropism assay is preferred to determine HIV-1 co-receptor usage. ”[Evidence level AI] “A genotypic tropism assay should be considered as an alternative test to predict 2013-05-08 · In parallel, tropism was inferred genotypically from the corresponding V3-loop sequences using Geno2Pheno[coreceptor] (5–20% FPR) and webPSSM-R5X4.

A co-receptor tropism assay should be performed whenever the use of a CCR5 co-receptor antagonist is being considered (AI). Co-receptor tropism testing is recommended for patients who exhibit virologic failure on a CCR5 antagonist (BIII). A phenotypic tropism assay is preferred to determine HIV-1 co-receptor usage (AI). Coreceptor tropism is defined as the ability of a particular HIV-1 virus to infect a target cell using a specific coreceptor. HIV requires two binding events to enter into a cell.
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Possible tropism results are R5, DM, X4, and X4 near the limit of detection (NLOD).

HIV-1 Coreceptor Tropism, Proviral DNA - The use of CCR5 antagonists requires screening for viral tropism to exclude patients harboring X4 or D/M virus.
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Su Z, Gulick RM, Krambrink A, et al. Response to vicriviroc in treatment-experienced subjects, as determined by an enhanced-sensitivity coreceptor tropism assay: reanalysis of AIDS clinical trials group A5211. J Infect Dis 2009; 200:1724.

A phenotypic tropism assay is preferred to determine HIV-1 co-receptor usage (AI). A genotypic tropism assay should be considered as an alternative test to predict HIV-1 co-receptor usage (BII).